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ytsJ [2017-8-11 16:0:3]
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ytsJ [2017-8-11 16:0:3]

malic enzyme, forms a transhydrogenation cycle with MalS for balancing of NADPH
locus
BSU29220
pI
5.05
mw
43.51 kDa
protein length
410 aa Sequence Blast
gene length
1230 bp Sequence Blast
function
malate utilization
product
NADP-dependent malate dehydrogenase
essential
no
ec
1.1.1.38
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • Gene

    Coordinates
    2,989,900 → 2,991,132

    Phenotypes of a mutant

  • Poor growth with malate as single carbon source [Pubmed|16788182]

    • The protein

    Catalyzed reaction/ biological activity

  • (S)-malate + NADP+ = pyruvate + CO2 + NADPH (according to Swiss-Prot) malate <-→ pyruvate

    • Protein family

  • malic enzymes family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|160EBB7885D7C7FD30A6E35605A862C156E2DA80|MleA]

    • Structure

  • [PDB|1WW8] (from ''Pyrococcus horikoshii'', 46% identity, 63% similarity)

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ]
    description
    [Pubmed|9387221]
    view in new tab

    genes
    [gene|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|dnaE]-[gene|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [PubMed|14593098,16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

    • regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A176 (ytsJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/176 NBRP B. subtilis, Japan]
  • GP612 (spc), available in [SW|Jörg Stülke]'s lab
  • GP1143 (spc), available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1432 (spc, based on [SW|pGP1870]), available in the [SW|Stülke] lab

    • FLAG-tag construct

  • GP1131 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab [pubmed|20933603]

    • Labs working on this gene/protein

  • [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

  • 16479537,16788182,12949160,9387221,23136871,22740702,15378759